England captain Ben Stokes and bowler Gus Atkinson have both been left out of the second Test against New Zealand that starts next week following an investigation into an incident in a London nightclub, the ECB said on Wednesday.
Former skipper Joe Root will lead the team at The Oval in the absence of Stokes.
"The England & Wales Cricket Board can confirm that, given the ongoing investigation, Ben Stokes and Gus Atkinson have not been made available for selection for the second Test against New Zealand, which starts at the Kia Oval on Wednesday 17 June."
Stokes and pace bowler Atkinson broke the team's midnight curfew in the early hours of Monday morning and were reportedly present when a member of England's security staff was involved in an incident with a Saracens rugby player.
It is the latest incident to involve a member of the England team after vice-captain Harry Brook was punched by a bouncer outside a nightclub in Wellington in October on the eve of a one-day international against New Zealand.
During the Ashes series in Australia, videos also emerged on social media showing England players inebriated during a mini-break in the coastal town of Noosa.
Accusations of a "drinking culture" were played down by Rob Key, England's managing director of cricket, in March but the ECB have introduced a strict midnight curfew.
England enjoyed a 115-run victory in the first Test, with Atkinson taking five wickets in New Zealand's second innings, but that positive result has now been overshadowed by another embarrassing incident for the ECB.
CRICKET REGULATOR CONDUCTING SEPERATE INVESTIGATION
The Cricket Regulator - a separate body from the ECB - is also conducting an investigation, the ECB said.
Stokes' long-term future is now under a cloud, but former captain Michael Vaughan believes he should not be dismissed.
"Yes, Ben Stokes broke a curfew. Yes, he made a mistake. But is that a sacking offence as England's Test captain? I don't think so," Vaughan told the Daily Telegraph.It is not the first time 35-year-old Stokes has found himself in trouble for off-field incidents.
In 2017, he was involved in a fracas outside a nightclub in Bristol that led to a criminal trial, at which he was later found not guilty of affray. He missed the 2017-18 Ashes tour before being cleared the following summer.
The latest episode marks another setback for England, who faced intense scrutiny about their discipline after the 4-1 Ashes defeat by Australia.
RENEWED PRESSURE ON COACH
It will also add renewed pressure on head coach Brendon McCullum, who retained his role across all formats in March following an ECB review, despite lingering concerns about the team's culture.
With vice-captain Brook overlooked, Root will now lead the team, having done so for a record 64 tests between 2017 and 2022.
The 35-year-old, who is England's all-time leading run-scorer, stepped down after a sequence of one win in 17 Tests and was succeeded by Stokes.
Pace bowler Jofra Archer and batter Jordan Cox have been added to the England squad that won the first Test at Lord's.
ENGLAND SQUAD:
Joe Root (captain), Rehan Ahmed, Jofra Archer, Sonny Baker, Shoaib Bashir, Jacob Bethell, Harry Brook, Jordan Cox, Ben Duckett, Matthew Fisher, Emilio Gay, James Rew, Ollie Robinson, Jamie Smith, Josh Tongue.