Bomb disposal teams had been sent to the Barcelona Sants station.
"We have received notification about 8 am (0700 GMT). Security of (rail company) Adif has seen an object with a scanner the shape of a possible explosive device inside a suitcase. We continue to make checks," Catalan police said on Twitter.
Two trains were evacuated.
Police closed two train lines in Barcelona's main station on Wednesday after a security scanner detected an object inside a suitcase that could be an explosive.
