Qatar Airways said it would "enforce" the new rules governing entry to the US following President Donald Trump's temporary order restricting arrivals from seven Muslim countries.
A spokesperson for the Gulf carrier said it would only carry passengers to the US who had the correct documentation.
"We are enforcing the new rules," said the official.
"If travellers to the US don't have the proper documentation, we are not going to take them to the US."
The news comes after Trump imposed tough new controls on travellers from Iran, Iraq, Libya, Somalia, Sudan, Syria and Yemen.
The newly inaugurated US president signed a sweeping executive order on Friday to suspend refugee arrivals and impose tough controls on travellers from the seven countries.
The airline, which flies to some 15 American cities, also posted a "travel alert" online, listing the paperwork required by citizens of the seven countries.
These include the permanent resident card, known as the "Green Card", and up to eight different types of visa. These are A1 & A2 (government officials and immediate family), C2 (travel to UN), G1 & G2 (representative and employees of international organisations), G3 & G4 (representatives to and employees of international organisations), and Nato.
Qatar Airways destinations in the US include New York, Atlanta and Chicago.