Vanessa Trump filed for divorce from US President Donald Trump's eldest son in a Manhattan court on Thursday, according to US media.
The 40-year-old is seeking an uncontested divorce to end her 12-year marriage with Donald Trump Jr., the New York Times reported.
"After 12 years of marriage, we have decided to go our separate ways," the couple said in a joint statement.
"We will always have tremendous respect for each other and our families. We have five beautiful children together and they remain our top priority. We ask for your privacy during this time," the statement added.
The couple were introduced to each other by the president at a 2003 fashion show. They married in 2005 at Mar-a-Lago, the Trump family's club in Florida's Palm Beach.
Donald Trump Jr., 40, has been leading the Trump Organisation's businesses with his brother Eric, 34, since their father entered office.
Last month, Vanessa Trump was taken to hospital when she told police she had been exposed to white powder while opening an envelope addressed to her husband.
There was no indication she suffered any injuries.
Reuters adds: In recent months, Trump Jr. has become enmeshed in an investigation of possible collusion between his father's presidential campaign and Russia in the 2016 presidential election. The president has denied any such collusion.
Trump Jr. arranged a 2016 meeting between a group of Russians and members of his father's campaign after an intermediary said the Russians offered damaging information about Democratic presidential candidate Hillary Clinton. "I love it," Trump Jr. responded to the intermediary in an email.
He has played a key role in the Trump Organisation since his father entered the White House in January 2017. The president said he would maintain ownership in his hotels, golf courses and hundreds of other businesses but hand off control to his two oldest sons.