The women, many of them dressed in white, descended through the arid hills leading to the river, where they erected a "peace tent".
US President Donald Trump on Wednesday said he believes Israelis and Palestinians are both willing to make a peace deal, adding that he does not think it will be as difficult as people thought.
Palestinian leader Mahmoud Abbas said on Thursday he hopes recent meetings with US President Donald Trump's team lead to "peace" between Israelis and Palestinians.
French President Francois Hollande said an international conference due in late May in Paris to relaunch peace talks between Palestinians and Israelis had been postponed but would take place this summer.
Troops detained 10 Israelis on Saturday as they joined a commemoration in the flashpoint West Bank city of Hebron for 29 Palestinian worshippers killed by a Jewish extremist in 1994.